微生物转谷氨酰胺酶生产菌株的筛选和鉴定
首发时间:2015-01-23
摘要:详细介绍了从土壤中分离筛选产转谷氨酰胺酶菌的方法,利用蛋白质交联絮凝进行初筛,通过酶活法复筛确定初筛菌是否产转谷氨酰胺酶微生物,并用16SrDNA序列进行菌种鉴定。通过筛选及鉴定,本研究得到两株产转谷氨酰胺酶菌生产菌株。?????
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Screening and indentification of Transglutaminase-producing Microorganism
Abstract:Detailed introduces the methods of isolation and screening of transglutaminase-producing microorganism from the soil, the cross-linking-flocculation precipitate method is used to select actinomycetes initially, measure the enzyme activity in order to ensure the strains produce transglutaminase.Afterwards, the strain were identified by the sequence of 16SrDNA . Through screening and identification, this study obtained two strains of transglutaminase-producing microorganism.????
Keywords: transglutaminase screening identification 16SrDNA identification
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No.4629835103090114****
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