补充谷氨酰胺可部分逆转超负荷训练诱导的大鼠巨噬细胞功能损害

肖卫华; 陈佩杰; 董静梅; 王茹; 罗贝贝

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Dietary glutamine supplementation partly reverses impaired macrophage function resulting from overload training in rats

首发时间：2015-04-17

XIAO Weihua ¹
XIAO Weihua (1981-), male, associate professor, field of research: excercise immunology
CHEN Peijie ¹
CHEN Peijie (1962-), male, professor, field of research: excercise immunology.
Jingmei Dong ¹ Ru Wang ¹ Beibei Luo ¹

1、College of Exercise and Sport Science, Shanghai University of Sport, Shanghai,200438

Abstract：The aim of this study was to evaluate the effect of overload training on the function of peritoneal macrophages in rats, and to test the hypothesis that glutamine in vivo supplementation would partly reverse the eventual functional alterations induced by overload training in these cells. Forty male Wistar rats were randomly divided into 5 groups: control group (C), overload training group (E1), overload training and restore one week group (E2), glutamine-supplementation group (EG1), and glutamine-supplementation and restore one week group (EG2). All rats, except those placed on sedentary control were subjected to 11 weeks of overload training protocol. Blood hemoglobin, serum testosterone and corticosterone of rats were measured. Moreover, the functions (chemotaxis, phagocytosis, cytokines synthesis, reactive oxygen species generation) of peritoneal macrophages were determined. Data showed that blood hemoglobin, serum testosterone, corticosterone and body weight in the overload training group decreased significantly as compared with the control group. Meanwhile, the chemotaxis capacity (decreased by 31%, p=0.003), the phagocytosis capacity (decreased by 27%, p=0.005), the reactive oxygen species (ROS) generation (decreased by 35%, p=0.003) and the cytokines response capability of macrophages were inhibited by overload training. However, the hindering of phagocytosis and the cytokines response capability of macrophages induced by overload training could be ameliorated and reversed respectively, by dietary glutamine supplementation. These results suggest that overload training impairs the function of peritoneal macrophages, which is essential for the microbicidal actions of macrophages. This may represent a novel mechanism of immunodepression induced by overload training. Nonetheless, dietary glutamine supplementation could partly reverse the impaired macrophage function resulting from overload training.

keywords： glutamine overload training macrophages function

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补充谷氨酰胺可部分逆转超负荷训练诱导的大鼠巨噬细胞功能损害

肖卫华 ¹
XIAO Weihua (1981-), male, associate professor, field of research: excercise immunology
陈佩杰 ²
CHEN Peijie (1962-), male, professor, field of research: excercise immunology.
董静梅 ² 王茹 ² 罗贝贝 ²

1、上海体育学院，运动科学学院，上海200438
2、College of Exercise and Sport Science, Shanghai University of Sport, Shanghai,200438

摘要：本文研究的目的是评价超负荷训练对大鼠腹膜巨噬细胞功能的影响，验证补充谷氨酰胺可能部分逆转超负荷训练导致的巨噬细胞功能损害这一假设。我们将40只wistar大鼠随机分成5组，对照组（C），超负荷训练组（E1）, 超负荷训练后恢复一周组（E2），谷氨酰胺补充组（EG1），谷氨酰胺补充和训练后休息一周组（EG2）。除对照组外，所以大鼠接受11周的超负荷训练。我们检测了大鼠的血红蛋白、血清睾酮和血清皮质醇，还测定了腹膜巨噬细胞的功能（趋化性、吞噬作用、细胞因子的形成、活性氧类的生成）。数据表明，和对照组相比，超负荷训练组大鼠血红蛋白、血清睾酮、皮质醇、体重都明显降低。此外，超负荷训练可抑制巨噬细胞趋化作用（降低了31%，p=0.003），吞噬能力（降低了27%，p=0.005），活性氧的产生（降低了35%，p=0.003）和炎性因子应答能力。但超负荷训练所诱导的巨噬细胞吞噬功能障碍和巨噬细胞细胞因子反应能力降低，可以通过膳食补充谷氨酰胺得到改善和逆转。这些结果表明，过度训练损害了巨噬细胞的杀菌能力，这可能是过度训练引起免疫抑制的一种新机制，而膳食补充谷氨酰胺能够部分逆转超负荷训练诱导的巨噬细胞功能受损。

关键词：谷氨酰胺超负荷训练巨噬细胞功能

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XIAO Weihua,CHEN Peijie,Jingmei Dong,et al. Dietary glutamine supplementation partly reverses impaired macrophage function resulting from overload training in rats[EB/OL]. Beijing:Sciencepaper Online[2015-04-17]. https://www.paper.edu.cn/releasepaper/content/201504-279.

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论文编号	201504-279
论文题目	补充谷氨酰胺可部分逆转超负荷训练诱导的大鼠巨噬细胞功能损害
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