Correlation analysis of peripheral blood TSHR,PKA and NIS mRNA expression in lactating women

• 1、Institute of Iodine Defiviency Disorders,Center for Disease Control and Prevention,Harbin Medical University,Harbin 150081

Abstract：Objective:To explore the role of the TSH-TSHR-cAMP-PKA signaling pathway in thyroid disease of lactating women,we detected the expression changes of the TSH-TSHR-cAMP-PKA signaling pathway related genes mRNA in peripheral blood .Methods:A total of 101 samples of peripheral blood of breast-feeding women were collected in Shanxi province's sufficient iodine and high iodine areas.There are 51 cases of thyroid dysfunction and 48 healthy controls.The mRNA expression of TSHR,PKA and NIS were detected by real-time quantitative PCR.Results:The mRNA expression of TSHR,PKA and NIS in case group (3.57±6.68,.59±2.43,.77±4.21)were higher than that of control group(1.77±2.89,1.16±1.31,1.69±4.89),but the differences were not statistically significant(P>0.05).The TSHR and the NIS in the case group and control group were significantly positively correlated((r（d）=0.862，P<0.01；r(c)=0.459，P<0.01);The PKA and the NIS in the case group was a significant positive correlation (r（d）=0.452，P<0.01),but in the control group there was no significant correlation(P>0.05);The TSHR had no significant correlation with the PKA.The TSHR mRNA expression in waterborne high iodine areas was significantly lower than the sufficient iodine areas,the difference was statistically significant(P<0.05);the PKA and NIS mRNA expression in high iodine area were lower than the sufficient iodine regions, the difference was not statistically significant (P> 0.05).Conclusion:Lactating women may be to protect themselves and their babies through the TSH-TSHR-cAMP-PKA signaling pathway regulating the iodine levels.

Keywords： Thyroid disease Lactating women Peripheral blood Signaling pathways TSHR PKA NIS