Isolation of Endophytic Fungi from Coptis chinensis Franch and Screening Activities Against Pathogenic Fungi

• 1、College of Plant Science, Jilin University, Jilin Changchun 130062

Abstract：Background: The endophytes of medicinal plants are one of the effective ways for screening bioactive ingredients and leading compounds. The strain of Cc-3 is endophytic fungi isolated from the medicinal plant Coptis chinensis and we are exploring the inhibition effect of Cc-3 against the plant pathogens, laying a foundation for the biological control of plant disease. Methods: By using the methods of confrontation and clasping for the determination of antifungal activity about Cc-3 against Setosphaeria turcica, Sclerotinia sclerotiorum, Aspergillus fumigatus, Fusarium oxysporum, Magnaporthe Oryaze, Cercospora zeae-maydis, Fusarium oxysporum, Cylindroca-rpon destructans and Botrytis cinerea and so on. Using headspace solid phase microextraction (HS-SPME), gas chromatography and mass spectrometry(GC-MS) is used to identify the volatile active substances by Cc-3. The strain of Cc-3 was identified by morphological and molecular biological methods. The results show that the strain of Cc-3 has strong antifungal activity against a variety of plant pathogens, especially Setosphaeria turcica and Sclerotinia sclerotiorum and the volatile active substance is tetramethylbenzene producing; The strain of Cc-3 was Phoma multirostrata.Conclusion: The Cc-3 strain of Phoma multirostrata isolated from the medicinal plant Coptis chinensis had better inhibition effect against etosphaeria turcica and Sclerotinia sclerotiorum and it is a potential biological control strain of plant disease .

Keywords： Endophytic fungi；Antifungal activity；Pathogenic Fungi；Biological