VPA对3T3细胞中多能性转录因子和miR-367的影响研究
首发时间:2015-06-26
摘要:目的 探讨VPA对microRNA和多能性转录因子的影响。方法 用MTT法确定VPA的浓度梯度,采用流式细胞术检测VPA对3T3细胞周期的阻滞情况,同时观察细胞形态的变化,并用荧光定量PCR法检测 Oct4和Sox2以及miR-367表达量的变化。结果 VPA剂量依赖性地抑制3T3细胞的增殖,且随着浓度的升高抑制作用增强,并将细胞周期阻滞在G2/M期。此外,VPA能使Oct4和miR-367的表达量增加。结论 VPA在体细胞重编程中可能介导多能性转录因子Oct4和miR-367的表达来促进重编程效率。
关键词: 细胞生物学 丙戊酸(VPA) 重编程 转录因子 miR-367
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Effect of VPA on the expression of transcription factor and miR-367 in 3T3 cells
Abstract:Objiective In this paper, we have investigated the effect of VPA on the expression levels of microRNA and pluripotency transcription factors. Methods We have determined the VPA concentration gradient by MTT assay, and detected the arrest of 3T3 cell cycle by flow cytometry, and observed the morphology of cells,and also detected the expression levles of Oct4 and Sox2 and miR-367 by fluorescence quantitative PCR method. Results The VPA dose-dependently inhibited the proliferation of 3T3 cells, and the inhibition rate were enhanced with increasing concentration,and cell cycle were arrested in G2/M phase. In addition,VPA can increase the expression of Oct4 and miR-367. Conclusion These results suggest that VPA may mediate the pluripotency transcription factors Oct4 and miR-367 to enhance the reprograming efficiency.
Keywords: cell biology VPA reprogramming transcription factor miRNA-367
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No.4648149943183143****
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