茶树芽叶紫化过程的转录组分析
首发时间:2015-07-10
摘要:应用新一代高通量测序平台Illumina HiSeqTM2500, 对'紫鹃'茶进行转录组测序和数据denovo组装,并对得到的unigene进行功能注释、分类及代谢通路分析。 结果显示,总共得到107,984条Transcripts和34,685条Unigenes。筛选出2,250个差异基因,其中上调表达1,270个,下调表达980个。通过对数据的生物信息学分析,筛选到27个差异基因与茶叶紫化过程相关的关键基因,其中10个基因直接参与了花青素合成,13个基因参与碳水化合物代谢,1个基因参与花青素的转运和3个基因参与油菜素类脂代谢过程。本研究揭示了茶树紫化过程中花青素代谢的调控特征。
关键词: 紫鹃茶 转录组 Illumina测序 花青素
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Transcriptome analysis of the purple process of tea shoots
Abstract:The transcriptome of Camellia sinensis var.kitamura was sequenced by using Illumina technology, and the functional annotations and metabolic pathways analyses of some unigenes were conducted in this present paper. In this study, 107,984 pieces of transcripts and 34,685 pieces of Unigenes were received. 2,250 differentially expressed genes were screened, among which 1,270 genes were up-regulated and 980 were down-regulated. Through bioinformatics data analysis, 27 key differentially expressed genes associated with the tea purple process were determined, among which 10 genes were involved in anthocyanin synthesis, 13 in carbohydrate metabolism, 3 in brassinosteroids, and 1 in anthocyanin transport. Therefore, these results provide the basis to understand the process of tea purple and metabolic characteristics of tea anthocyanins.
Keywords: Camellia sinensis var. kitamura Transcriptome Illumina sequencing Anthocyanin
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No.4649532518195143****
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