铵态氮对茶树扦插过程中根系形态及其生理生化影响研究
首发时间:2015-11-12
摘要:为研究不同浓度铵态氮对茶树扦插生根的影响,本试验采用砂培的方式对茶树插穗进行生根诱导,测定了不同铵态氮浓度下根系发育过程中的形态及生理生化变化。研究发现,插穗生根时间相差不大,但低氮处理的不定根数目最多,愈伤率和生根率最大,而高氮处理根系最长。在扦插0-20d三个处理的含水量、可溶性蛋白、可溶性糖、淀粉含量、总氮及C/N比值方面差异并不显著。在扦插20d-60d不同处理变化较大,低氮处理含水量、可溶性蛋白、可溶性糖、淀粉含量及C/N比值均显著高于高氮处理,而高氮处理的总氮含量则显著高于低氮处理。在酶活性方面,低氮处理中POD酶及PPO酶活性高于高氮处理。因此,适当降低铵态氮浓度有利于促进茶树插穗根系的发育。
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Study on the effect of ammonium nitrogen on morphology and physiology and biochemistry of tea root on the process of cuttage
Abstract:The effects on cuttage rooting of tea tree in different concentrations of ammonium nitrogen was studied in the sand culture. The changes of morphology and physiology and biochemistry were determined during the development process in different concentrations of ammonium nitrogen. As a result, the time of cuttage rooting had little difference among the treaments, but the number of adventitious root, callus rate, rooting rate were the highest in the low N treatment, however, the root length was the highest in the high N treatment. The content of water, soluble protein, soluble sugar, starch, total N, C/N had no significant differences during the 0 to 20 days, but the index of low N treatment were significantly higher than the high N treatment except total N, whie the total N was highest in the reatment high N treatment during the 20 to 60 days. The enzyme activity of POD and PPO in the low N treatment were higher than the high N treatment. Therefore, the developmengt of cuttage rooting of tea tree could be promoted by properly reduce the content of ammonium nitrogen.
Keywords: Ammonium nitrogen Camellia sinensis (L.) root morphology;physiological and biochemical
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铵态氮对茶树扦插过程中根系形态及其生理生化影响研究
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