MIIP抑制子宫内膜癌细胞迁移及侵袭
首发时间:2015-11-18
摘要:目的:探讨MIIP基因对子宫内膜癌(EC)细胞迁移及侵袭能力的影响。 方法: Western blot 检测MIIP在EC细胞株AN3CA、ECC-1、Ishikawa、HEC1A及HEC1B中的表达。Si-RNA及Ad-MIIP调节EC细胞MIIP表达。Transwell转移、侵袭实验检测MIIP下调/上调前后对EC细胞迁移、侵袭能力的影响。 结果:各细胞株中,HEC1A细胞MIIP表达水平相对较高,Si-RNA能使HEC1A细胞中MIIP表达水显著下降;MIIP在AN3CA及HEC1B细胞株中几乎不可见,Ad-MIIP感染后,AN3CA及HEC1B细胞中MIIP表达水平明显上升。Thanswell迁移培养体系中,HEC1A细胞MIIP下调后,穿膜细胞数较对照组明显增加;AN3CA及HEC1B细胞MIIP上调后,穿膜细胞数较对照组明显减低。同时,Thanswell侵袭培养体系中,HEC1A细胞MIIP下调后,穿膜细胞数也较对照组明显增加;HEC1B细胞MIIP上调后,穿膜细胞数较对照组明显减低。 结论:在MIIP不同表达水平的EC细胞株中,MIIP表达水平可以成功的上调或下调。HEC1A细胞MIIP下调后,细胞迁移、侵袭能力明显增加;恢复MIIP表达明显抑制了HEC1B细胞迁移、侵袭能力;同时抑制了AN3CA细胞迁移能力。因此,MIIP基因可抑制EC细胞的迁移、侵袭。?????
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MIIP inhibit endometrial carcer cell migration and invasion
Abstract:Objective: To investigate whether MIIP inhibited EC cell migration and invasion. Methods: Western blot was used to measure MIIP expression in EC cell lines including AN3CA、ECC-1、Ishikawa、HEC1A,and HEC1B. Si-RNA was used to knockdown MIIP in HEC1A cell. Adenovirus-MIIP was used to overexpress MIIP in HEC1B and AN3CA cell.The effect of MIIP on cell migration and invasion was measured by wound healing and Boyden Chamber transwell assay. Results: MIIP protein is detectable at low level in the HEC1A cell line but undetectable in the AN3CA and HEC1B cell lines. MIIP expression was significantly decreased in HEC1A through Si-RNA knockdown or increased in HEC1B and AN3CA through Ad-MIIP overexpression. Knockdown of MIIP expression with siRNA in HEC1A cells significantly increased cell migration when compared with control siRNA treated cells. Elevated expression of the MIIP significantly decreased cell migration in AN3CA and HEC1B cells compared with control empty vector-transfected cells. The invasion of the MIIP-knocked-down HEC1A cells was significantly increased compared with control. Consistently, cell invasion was significantly decreased in MIIP overexpressed cells compared with control HEC1B cells. Conclusions: We can successfully decrease or increase MIIP expression in EC cells. Knockdown of MIIP increased EC cell migration/invasion but restoration of MIIP inhibited EC cell migration and invasion.
Keywords: Endometrial carcinoma Migration and invasion inhibitory protein (MIIP) migration;invasion
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