The effect of 10-hydrocamptothecin on the peripheral blood mononuclear cell in rheumatoid arthritis

• 1、Peking University People's Hospital, Beijing 100044

Abstract：Objective To observe the effect of 10-hydrocamptothecin (HCPT) on the proliferation, apoptosis and cytokine production of peripheral blood mononuclear cell (PBMC) in rheumatoid arthritis (RA) and to explore the immunoregulatory mechanism of HCPT in the treatment of RA. Methods We cultured the PBMC in vitro and stimulated the cells by the different concentration of HCPT. Proliferation was assayed by 3H-TdR incorporation test. The level of cell apoptosis was measured by flow cytometry with Annexin V/FITC Staining (FCM) method. Transforming growth factor beta1 (TGF-β1), Interleukin (IL)-10 and tumor necrosis factor (TNF) -α in the cell culture supernatant were detected by ELISA kit. Results 1. Three concentration of HCPT could inhibit the proliferation of the PBMC in vitro and the effect was dose-dependent (p<0.05). 2. HCPT could induce apoptosis of PBMC (p<0.05). 3. The level of TGF-β1 secretion was significantly higher in the supernatant of PBMC stimulated with HCPT than that in the negative control. Conclusions HCPT could obviously inhibit the proliferation and induce the apoptosis of PBMC in RA, and HCPT could up-regulate the level of TGF-β1. HCPT might be an alternative choice in the treatment of RA.

Keywords： Rheumatology & Autoimmunity 10-hydrocamptothecin rheumatoid arthritis peripheral blood mononuclear cell proliferation apoptosis