Conformation and charge co-modulated ultrasensitive biosensor based on single nanopore channel

• 1、Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen,361005,China
• 2、 Department of Electronic Science,School of Physics and Mechanical & Electrical Engineering,Xiamen University,Xiamen,361005,China

Abstract：In this manuscript, we have demonstrated the proof-of-concept of the conformation and surface charge co-modulated ion transport inside a synthetic nanopore engineered by a single stimulus. We integrated an ATP binding aptamer (ABA) into a single glass conical nanopore channel and achieved the simultaneous transitions involving both conformation and charge densities upon the presence of ATP. The synergistic effect of pore size and surface charge has largely enhanced the response efficiency of nanopore. Based on this property, we further developed an ultrasensitive biosensing platform for the detection of ATP with a detection limit down to 0.36 pM (S/N=3).This biomimetic nanodevice exhibited excellent selectivity. This work not only facilitates a better understanding of the ionic transport processes in biological ion channels but also advances their real-world applications in biosensors and actuators.

keywords： Adenosine triphosphate Biosensor Conformation Charge Single glass conical nanopore channel

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