IL-13基因microRNA靶序列单核苷酸多态性与乳腺癌发病的关系
首发时间:2015-12-07
摘要:目的 探讨IL-13基因microRNA靶序列结合区域的单核苷酸多态性及环境因素交互作用与乳腺癌发病的关系。方法 采用病例对照研究方法,首先应用生物信息学软件对IL13基因靶序列SNPs进行预测,应用TaqMan探针分型技术检测乳腺癌患者和健康对照者外周血DNA中IL-13基因micro-RNA靶序列多态位点rs847(G-A)、rs848(G-T)、rs1295685(C-T)。应用叉生分析及多因素Logistic回归的方法分析IL-13基因多态性与环境因素的交互作用。结果 该三个SNPs位点不是乳腺癌发生的独立的危险因素,但与环境因素间有显著的联合作用及交互作用。3个SNPs位点突变基因型与每周食用隔夜食物大于3次有显著的联合作用,OR值分别为1.991(95%CI:1.423-2.784); 1.919(95%CI:1.372-2.684); 1.939(95%CI:1.390-2.704)。rs1295685位点突变基因型与大蒜的摄入有显著的拮抗作用,OR值为0.605(95%CI:0.377-0.97)。结论IL-13基因micro-RNA靶序列多态位点rs847、rs848、rs1295685与环境因素共同作用与女性乳腺癌的发病相关。
关键词: microRNA 靶序列 单核苷酸多态性 IL-13 乳腺癌
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The association between microRNA binding region single nucleotide polymorphisms in IL-13 gene and the risk of breast cancer
Abstract:Objective To investigate the role of single nucleotide polymorphisms (SNPs) within microRNA binding region of IL13 gene and its interaction with environment factors on breast cancer. Methods Based on the prediction of SNPs within microRNA binding region of IL13 gene by using bioinformatics software, a case-control study was designed and TaqMan probe genotyping technique was used to detect the genotypes of rs847 (G-A), rs848 (G-T) and rs1295685 (C-T) in peripheral blood DNA from breast cancer patients and healthy controls. Crossover study and multivariate logistic regression were used to analyze the interactions between IL-13 gene polymorphisms and environment factors. Results These three SNPs were not independent risk factors of breast cancer. However, there were significant combining effects of these three SNPs with the over-night food consumption(≥3 times/week), the ORs value were 1.991 (95%CI: 1.423-2.784), 1.919 (95%CI: 1.372-2.684), 1.939 (95%CI: 1.390-2.704) respectively. Also, a significant antagonistic effect of rs1295685 and garlic was found with the OR as 0.605 (95%CI: 0.377-0.970). Conclusions Polymorphisms of rs847, rs848, and rs1295685 in IL-13 micro-RNA binding region interact with environment factors and contribute to the development of brest cancer.
Keywords: microRNA-binding site single nucleotide polymorphism IL-13 breast cancer
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