系统性红斑狼疮患者与健康对照血浆中microRNA的表达差异
首发时间:2015-12-07
摘要:本研究目的为筛查并验证系统性红斑狼疮(SLE)患者的血浆microRNA(miRNA)表达谱,探讨miRNA的表达与SLE发病及其病程的关联性。采用病例对照研究,利用miRNA芯片检测血浆miRNA的表达水平,并利用Real-time PCR方法进行独立验证,收集纳入对象的人口统计学资料和临床资料。芯片筛查结果显示,SLE患者与对照相比,有158个miRNA表达有差异,其中上调有32个,下调有126个。扩大样本量,验证发现miR-16-5p, miR-101-3p 和miR-25-3p在两组之间差异有统计学意义,与芯片筛查预测的结果一致。结果还发现SLE患者miRNA的表达水平与某些临床症状或实验室指标之间也有关联。总体来讲,病例与对照组间miRNA的表达差异显示miRNA可能与系统性红斑狼疮有关联。
关键词: 流行病学; 微RNAs; 血浆;红斑狼疮 系统性
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Plasma microRNAs profiles in patients with systemic lupus erythematosus
Abstract:Background: The aim of this study was to establish a plasma microRNAs (miRNAs) profile which detected by miRNA microassay so as to identify early diagnostic biomarkers in patients with systemic lupus erythematosus (SLE). Methods: Two panels were recruited for this study. The miRNA expression in plasma was detected by miRNA microarray. Palsma samples were collected from 30 patients with SLE and 30 healthy controls. The second phase, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to further confirm the microarray data in 37 patients and 39 controls. 2-△Ct was used to compute the expression value of confirmed miRNAs . P values (2-tailed) less than 0.05 were considered statistically significant. Results: Compared to healthy controls, 32 up-regulated and 126 down-regulated miRNAs were detected in the plasma from patients with SLE. Five miRNAs (miR-25-3p, miR-101-3p, miR-16-5p, miR-664a-3p, miR-744-5p) were chosen to confirm by qRT-PCR. The result of miR-25-3p, miR-101-3p and miR-16-5p were nearly consistent with previous data from miRNAs microarray. But there was no significant difference for the expression of miR-664a-3p and miR-744-5p between patients and controls. Conclusions: Aberrantly expressed plasma miRNAs may be used as biomarker candidates for minimally invasive diagnosis of early patients with SLE in the future.
Keywords: Epidemiology MicroRNAs Plasma Lupus erythematosus, Systemic
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系统性红斑狼疮患者与健康对照血浆中microRNA的表达差异
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