Fluorescent carbon dots derived from lactose for assaying folic acid
首发时间:2015-12-08
Abstract:The fluorescent carbon dots were successfully synthesized by simply heating the mixture of lactose and NaOH solution. The as-synthesized carbon dots had been systematically characterized by fluorescence, FTIR, HR-TEM and 13C-NMR. Since the fluorescence of the carbon dots were efficiently quenched by folic acid, the carbon dots were employed as selective fluorescence probes for detecting of folic acid, depending on the formation of hydrogen bond among the functional group of folic acid (-OH, -COOH and -NH2) and -OH and -COOH of the carbon dots. Moreover, the decrease of fluorescence intensity was capable of detecting folic acid in a linear range of 6×10-5 mol/L-8×10-8mol/L with a detection limit of 1.2×10-9 mol/L at a signal-to-noise ratio of 3, suggesting a promising assay for folic acid. Significantly, the practicability of this fluorescence probe to assay folic acid in human urine samples was further evaluated.
keywords: Pharmaceutical Analysis Carbon Quantum dots Fluorescence quenching
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以乳糖为碳源合成碳点并用于叶酸的分析检测
摘要:本文以一步法利用乳糖为碳源成功合成了荧光碳点,并用荧光、紫外、红外、高倍透射电镜和核磁共振碳谱对其进行系统表征。结果表明其表面含有羧基羟基等官能团,可以特异性地与叶酸的羟基、羧基和氨基形成氢键,从而导致碳点荧光信号的猝灭。因此,碳点作为荧光信号探针被用于叶酸的选择性分析检测中。其检测限低至1.2×10-9 mol/L,具有良好的线性范围(6×10-5 mol/L-8×10-8mol/L ) .最后,还将其用于人体尿液实际样的检测,结果证明了其在实际分析应用中的潜能。
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