基于石墨烯纳米带修饰电极的嘌呤碱基和嘧啶碱基电化学传感
首发时间:2015-12-09
摘要:基于石墨烯纳米带(graphene nanoribbons , GNRs)修饰的玻碳(GC)电极具有好的电催化活性和宽的电位窗口,开展了嘌呤碱基(鸟嘌呤(G)和腺嘌呤(A))和嘧啶碱基(胸腺嘧啶(T)和胞嘧啶(C))的电化学传感研究。与裸玻碳电极和碳纳米管修饰玻碳电极相比,GNRs修饰玻碳电极对G、A、T和C四个碱基的电化学传感具有检测限低、灵敏度高、线性范围宽的特点;对G、A、T和C四碱基共存体系, G和A、A和T、T和C在GNRs/GC电极上的峰电位差分别达到290 mV、200 mV、140 mV,表明GNRs/GC电极可以实现在四元共存体系中同时检测G、A、T和C四个碱基。此外,GNRs/GC电极具有优良的稳定性、重现性和抗干扰能力,并且可成功应用于单核苷酸多态性检测。
关键词: 分析化学 电化学传感 石墨烯纳米带 DNA碱基 单核苷酸多态性
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Electrochemical oxidation of purine and pyrimidine on the graphene nanoribbons modified electrode
Abstract:Based on the wide potential window and good electrochemical properties of the modified electrode, the direct electrochemical oxidation of four free DNA bases on the graphene nanoribbons(GNRs) modified glassy carbon (GC) electrode was achieved. Compared with bare GC and carbon nanotubes (CNTs) modified electrodes, the GNRs/GC electrode exhibited obviously enhanced electrocatalytic activities towards the oxidation of guanine (G), adenine (A), thymine (T) and cytosine (C). Under the optimum conditions, the GNRs/GC electrode has low detection limit, high sensitivity and wide linear range for the detection of G, A, T and C. On the other hand, the electrochemical oxidation of quaternary mixture of G, A, T, and C on the GNRs/GC electrode was investigated. The values of the peak potential difference between G and A, A and T, T and C are 290, 200, 140 mV, respectively. The peak separations are large enough for their recognition in mixture and simultaneous detection of G, A, T and C. The GNRs/GC electrode also displayed good stability, reproducibility and excellent anti-interferent ability, and good capability for the analysis of Single-Nucleotide Polymorphisms (SNPs) without hybridization or labeling.
Keywords: analytical chemistry electrochemical sensing graphene nanoribbons DNA bases Single-Nucleotide Polymorphisms
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基于石墨烯纳米带修饰电极的嘌呤碱基和嘧啶碱基电化学传感
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