Identification and analysis of a novel dwarfing gene in three Thinopyrum elongatum germplasm lines

• 1、The national center for wheat improvement taian sub-center, Taian, Shandong, China, 271018
• 2、Shandong Agricultural University State key laboratory of crop biology, Taian, Shandong, China, 271018
• 3、 The national center for wheat improvement taian sub-center, Taian, Shandong, China, 271018

Abstract：Thinopyrum elongatum is an important relatives of wheat. We intended to indentify three dwarfing germplasm lines which were selected from filial generation of wheat and Thinopyrum elongatum by cytology and in situ hybridization. As the results indicating that the number of chromosome of Shannong31504 is 44 and it has cytological stability, which indicate that it is a disomic alien addition line; the number of chromosome of Shannong 31504-1 and 31504-3 are 42 and they also have cytological stability. GISH was proceed using genomic DNA from Pseudoregnaria strigosa(St) as the probe concluding that the two alien chromosomes of Shannong31504 belonged to St genome, Shannong31504-1 and 31504-3 were wheat-Thinopyrum elongatum alien translocation with little fragmentation of Thinopyrum elongatum. FISH with the probe of pTa535 and pSc119.2 was used to establish the genome constitution of Shannong 31504-1 and 31504-3. We found that their 6B and 1D chromosomes have the same structural mutation. The results indicateing that they are the same wheat-Thinopyrum elongatum introgression lines, and the structural mutation may occur on 6B and 1D chromosomes.?????

Keywords： Wheat Thinopyrum elongatum Dwarf gene Chromosome in situ hybridization