Preparation of highly active peptides from Spirulina mud protein hydrolysate

• 1、College of Life Science and Technology,Huazhong Agricultural University,Wuhan,430070

Abstract：The preparation of highly active peptides from Spirulina mud by the enzyme which produced by pichia fermentation was studied. The orthogonal experiment was used to study the hydrolysis conditions. The influence factors, including temperature, pH, the ratio of material to water, enzyme dosage and hydrolysis time, were investigated according to DH and TCA-SNI. The optimum hydrolysis conditions were as follows: the ratio of material to water 1:8, enzyme dosage 700 U/g, hydrolysis temperature 55℃, pH 8.5, hydrolysis time 3 h. Under the optimal conditions, the DH of the hydrolysates reached 11.8% and the TCA-SNI of the hydrolysates reached 55.13%.The molecular weight of enzymolysis product was determined by Gel filtration chromatography. The result showed that the oligopeptide which the molecular weight was less than 1000 Da reached 55.02%. And the small peptide mainly with 260 Da molecular weight composed by 2 amino acids reached 29.07%. The hydrolysates had abundant nutrients: protein 62.52%, total phosphorus 1.00%, calcium 0.13% and iron 803 mg/kg.

Keywords： Spirulina mud enzymolysis active peptides