超声辅助强化木质纤维素氨浸预处理过程研究
首发时间:2016-01-05
摘要:本文选择玉米芯为原料,设计了超声辅助氨浸预处理工艺,对处理后样品的组分含量与纤维素酶解转化率进行了考察并优化了工艺参数。在超声辅助氨浸预处理过程中,玉米芯中的纤维素组分含量基本保持不变而半纤维素则发生明显的提高,同时木质素含量明显减少。通过改变底物的组成,强化了氨浸脱木素效果,Medium Power超声6 min后的玉米芯样品可以提高34.2%的酶解率。通过对还原糖释放速率的动力学拟合可知,超声样品的还原糖释放速率为未超声样品1.3倍,同时最大还原糖浓度是未超声样品1.4倍,这说明超声强化的氨浸预处理可大幅提高玉米芯的酶解效率。
关键词: 化学工程 玉米芯 纤维素酶 超声 预处理 酶解糖化
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Enhanced Ammonia Pretreatment of Lignocellulose Aided by Ultrasonic Treatment
Abstract:Selecting corncob as raw materials, this paper designed the pretreatment process of ultrasonic assisted ammonia soaking, optimized the operating parameters and investigated its effects on the constituents of substrates and the subsequent enzymatic hydrolysis rate. Cellulose content remained unchanged in the pretreatment process, and there was a great improvement in hemicellulose content, while percentage of lignin decreased. Delignification effects were intensified by changing the constituents of substrates and the enzymatic hydrolysis rate improved 34.2% after 6 min of pretreatment with Medium Power using corncob. Data of kinetic fittings showed that the releasing rate of reducing sugars in pretreated substrates was 1.3 times higher than that of untreated materials and the maximum reducing sugars concentration improved 40% after pretreatment, which indicated that ultrasound-assisted pretreatment combined with ammonia leaching could significantly enhance subsequent enzymatic hydrolysis rate.
Keywords: Chemical engineering corncob cellulase ultrasonic pretreatment enzymatic hydrolysis
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