表面活性剂强化微晶纤维素和玉米芯酶解过程研究
首发时间:2016-01-06
摘要:本文重点考察多种表面活性剂对Avicel微晶纤维素和玉米芯酶解的影响规律。发现非离子表面活性剂可有效增强水解效率。当纤维素酶浓度为10FPU/g纤维素时,采用优化后的吐温80(浓度为0.1%)可使稀硫酸-氨水预处理的玉米芯酶解葡萄糖得率增加至78.5%,比无吐温80、纤维素酶浓度为30FPU/g纤维素(酶解率为65.0%)要高。结果表明:非离子型表面活性剂可显著增强酶解效率,降低酶用量。此外,还考察了表面活性剂对Avicel和玉米芯酶解过程中蛋白吸附动力学,发现吐温80可降低纤维素酶在非纤维素底物上的吸附,而对纤维素酶在纤维素上的吸附无明显作用。
关键词: 化学工程 玉米芯 微晶纤维素 纤维素酶 表面活性剂 酶解糖化
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Enhanced enzymatic hydrolysis of microcrystalline cellulose and corncob by surfactant
Abstract:In this paper, various surfactants were chosen to study their effects on enzymatic hydrolysis of both Avicel and corncob, showing that the hydrolysis efficiencies can be enhanced by non-ionic surfactant. Glucose yield from corncob treated by H2SO4-SAA pretreatment increased to 78.5% with the optimized Tween 80 concentration of 0.1% at cellulase loading of 10 FPU/g cellulose, which was higher than that (65.0%) without Tween 80 at 30 FPU/g cellulose. The results indicated that non-ionic surfactant could effectively increase the efficiencies of enzymatic hydrolysis and thus decrease enzyme usage. The adsorption kinetic of protein proved that cellulase adsorption on non-cellulosic substrate was weakened by Tween 80, whereas it has little effect on cellulase adsorption on cellulose.
Keywords: Chemical engineering corncob microcrystalline cellulose cellulase surfactant enzymatic hydrolysis
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No.4674334384743145****
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