麦冬多糖调控肥胖小鼠脂质代谢紊乱的代谢组学研究
首发时间:2016-02-02
摘要:目的:本研究基于代谢组学手段探究麦冬多糖MDG-1干预膳食诱导肥胖(Diet-induced obesity, DIO )小鼠脂质代谢紊乱可能的作用机制;方法:36只雄性C57BL/6小鼠按体重随机分成空白组、肥胖模型组和MDG-1干预组,实验持续16周,于实验终点收集各组小鼠肝组织样本。对肝组织进行超高效液相色谱-飞行时间质谱联用(UPLC/TOF-MS)的代谢组学分析,并采用偏最小二乘判别分析法(PLS-DA)对数据进行分析。同时,测定体重、累计摄食量、血脂水平等生化指标;结果:和模型组相比,MDG-1可有效控制肥胖小鼠体重增加,改善血脂水平。此外,MDG-1对肥胖小鼠肝组织代谢轮廓具有一定的回调作用,并发现了十三个潜在的生物标记物。结论:麦冬多糖MDG-1可以改善肥胖小鼠代谢紊乱,其作用机制可能与减少肝脏中胆固醇及胆汁酸的生物合成,增强酮体的生成有关。
关键词: 药理学 麦冬多糖MDG-1 脂质代谢紊乱 UPLC/TOF-MS 生物标记物
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Metabonomic study of MDG-1 on regulation of the lipid metabolism disorders in fat mice
Abstract:Objective: The research established a metabonomics-based method to investigate underlying mechanism of MDG-1 in preventing lipid metabolism disorders of fat mice. Method: 36 male C57BL/6 mice were randomly divided into three groups( control group, model group and MDG-1 intervened group ). After 16-weeks treatment, liver samples were analyzed by ultra-performance liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry( UPLC/TOF-MS ), to find out the potential identification biomarkers by partial least squares-discriminatory analysis( PLS-DA ). In addition, biochemical indices of body weight, food intake and blood lipid levels were collected. Result: Compared with model group, significant weight loss and improved lipid levels in MDG-1 group were achieved. In addition, hepatic metabonomic results exhibited that MDG-1 could reverse abnormally expressed metabolites. Meanwhile, thirteen potential biomarker were discovered. Conclusion: MDG-1 could regulate metabolism disorders in DIO model. Furthermore, the effect may be realized via restricting the biosynthesis of cholesterol as well as some secondary bile acids , and promoting the generation of ketone body.
Keywords: Pharmacology MDG-1 metabolism disorders UPLC/TOF-MS biomarker
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