制备以纳米金/石墨烯为基底的葡萄糖传感器
首发时间:2016-04-01
摘要:本文使用聚二烯二甲基氯化铵(PDDA)功能化石墨烯(G),使其表面带正电,与带负电的纳米金(AuNPs),进行静电自组装,制得AuNPs/G。为了验证这种材料的应用性,将其修饰到玻碳电极上,以葡萄糖氧化酶(GOD)作为模板。将制备的G/AuNPs/GOD/GCE作为葡萄糖传感器,其平均电子转移速率为1.64 s-1(7.71 s-1)。制备的葡萄糖传感器对葡萄糖的响应范围是1-9mM,检出限为0.1mM(S/N=3)。这种传感器能够有效的排除一般共存化合物的干扰,并且可以对血清样品进行测定,其回收率为在95.7%-107.6%。因此,可以说明G/AuNPs杂化材料为GOD提供了优良的导电性和适宜的微环境,这也为固定其他的酶或蛋白质提供一种有利的平台。
关键词: 石墨烯 AuNPs 石墨烯/AuNPs 葡萄糖传感器
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Preparation of graphene / gold nanoparticles-based glucose biosensing by the electrostatic self-assembly
Abstract:Functionalized by Poly(diallyldimethylammonlum chloride) ,the graphene(G) was positively charged.Then it was electrostatically self-assembled with negatively charged gold nanoparticles (AuNPs) , to obtain the compound material G/AuNPs. In order to verify the application of this material, we droped it to the glassy carbon electrode (GCE) , with glucose oxidase (GOD) as a template, a glucose biosensor of G/AuNPs/GOD/GCE was successfully developed.The average electron transfer rate is 1.64 s-1(7.71s-1).The glucose biosensor showed a good linear range from 1.0mM to 9.0mM, with a low detection limit of 0.1mM (S/N=3). What's more, this kind of biosensor successfully determined the serum sample, with the recovery being 95.7%-107.6%. Therefore, it's conclusioned that G/AuNPs compound material provided excellent electrical conductivite and suitavle microenvironment for GOD, which also provide a favorable platform for the immovilization of other enzymes or proteins.
Keywords: graphene AuNPs graphene/AuNPs glucose sensor
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