Study on liver protection of Wuling San and its mechanism in fructose-induced mice

• 1、School of Life Science, Nanjing University, Nanjing, 210023

Abstract：Objective：To investigate the protection of Wuling San against liver inflammatory injury and its mechanisms in fructose-induced mice. Methods: ICR mice were fed with 30% fructose in drinking water for 6 weeks. Then mice were orally administrated for next 6 weeks with water, Wuling San, positive drug allopurinol daily, respectively. Results: Wuling San significantly decreased lipid deposition in the liver of fructose-fed mice. It also inhibited hepatic inflammation and reduced serum and hepatic IL-1β levels in fructose-fed mice. Wuling San regulated hepatic insulin signaling, suppressed NF-κB signaling and NLRP3 inflammasome activation in the liver of fructose-fed mice. Conclusion: Wuling San was demonstrated to improve fructose-induced metabolic syndrome in mice. Wuling San also reduced hepatic triglycerides accumulation and inflammation in fructose-induced metabolic syndrome of mice, which may be associated with the regulation of hepatic insulin signaling, suppression of NF-κB signaling and NLRP3 inflammasome activation in this animal model. This study initially reveals liver protection mechanism by which Wuling San allevats inflammation and injury in fructose-induced metabolic syndrome of mice, and may provide scientific basis for the prevention and treatment of metabolic disorders-associated liver injury in the clinical.

Keywords： Wuling San Excess fructose intake Liver protection