梅花花朵香气物质形成的比较转录组分析
首发时间:2016-05-17
摘要:梅花(Prunus mume)蔷薇科李属植物,是一种非常理想的木本观赏植物。本研究采用Illumina HiSeqTM 2000 测序平台对梅花品种"三轮玉蝶"无香花蕾及盛开状态的花朵进行比较转录组测序,并对香气产生时期基因表达变化进行了分析,从而挖掘梅花花香形成的代谢过程与核心调控基因。测序结果共得到4.48G数据,分别有76.81%与61.43%的基因成功比对至已有基因组上。我们发现了花朵发育过成的两个时期中的差异基因共6954个(其中上调基因2156个,下调基因4798个)。对差异基因的GO注释,发现存在差异的基因功能集中于次级代谢、细胞组成等方面,而KEGG通路的注释表明苯丙烷/苯环类物质及次级代谢物质合成代谢通路基因有显著富集,在调控梅花花香物质合成过程中居主导地位。转录组中共鉴定出524个差异表达的转录因子。本研究通过转录组学技术初步分析了梅花花芽产生香气前后基因变化网络,为后期梅花花香调控基因探究、探索梅花花香形成机理及李属其他物种的香花育种提供可靠的理论依据。
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Comparative transcriptome profiling into the formation of floral scent in Mei flower
Abstract:Mei flower (Prunus mume) is an ideal woody ornamental plant among Prunus subfamilies of Rosaceae because of its inviting fragrance in winter. In this approach into the formation of floral scent, Illumina HiSeqTM 2000 was conducted to analyse the change of different genes. Approximately 44.8 million high-quality reads were obtained from two pooled cDNA library, of which 76.81% and 61.43% were mapped into reference genome. Digital gene expression (DGE) profiling analysis revealed 6954 differential expression genes (DEGs) during flower development. GO term classification and KEGG pathway analysis indicated that the levels of transcripts changed significantly in" metabolic process", including "benzenoid biosynthetic process"and pathways of "Phenylpropanoid biosynthesis","Biosynthesis of secondary metabolites". In addition, 524 TFs showed differential expression during flower development were digged out of this transcriptome. The transcriptome and DGE profiling provide an important resource for functional genomics studies and give us a dynamic view of biological process during flower development . These data lay the basis for elucidating the molecular mechanism of floral scent formation and regulation in Prunus subfamilies. The results also provide the opportunities for genetic modification of floral scent profile in Mei flower.
Keywords: Mei flower scent transcriptome benzenoid metabolism
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