Identification of microRNAs related to the genetic male sterility of Chinese cabbage using high-throughput sequencing
首发时间:2016-05-20
Abstract:MicroRNAs (miRNAs) negatively regulate gene expression, and play important roles in growth and development, cell proliferation, apoptosis, signal transduction, and in biotic and abiotic stress responses. In plants, miRNAs also play an important role in pollen development. However, to date, few pollen development-related miRNAs have been identified. In this study we used the fertile and sterile buds of an AB line of Chinese cabbage, 'AB03', as the test material, and constructed small RNA libraries. Using high-throughput sequencing, we screened 19 differentially expressed miRNAs, obtained 46 novel miRNAs and predicted 200 target genes of 30 miRNA families. Quantitative real-time reverse transcription PCR verified that the expressions of all 19 differentially expressed miRNAs were basically consistent with the sequencing results. Our study identified miRNAs that might participate in pollen development and provided the relationship between miRNAs and their target genes, which might form the theoretical basis for future research.
keywords: Chinese cabbage;genetic male sterility;pollen development;miRNA; high-throughput sequencing
点击查看论文中文信息
Identification of microRNAs related to the genetic male sterility of Chinese cabbage using high-throughput sequencing
摘要:MicroRNAs (miRNAs) negatively regulate gene expression, and play important roles in growth and development, cell proliferation, apoptosis, signal transduction, and in biotic and abiotic stress responses. In plants, miRNAs also play an important role in pollen development. However, to date, few pollen development-related miRNAs have been identified. In this study we used the fertile and sterile buds of an AB line of Chinese cabbage, 'AB03', as the test material, and constructed small RNA libraries. Using high-throughput sequencing, we screened 19 differentially expressed miRNAs, obtained 46 novel miRNAs and predicted 200 target genes of 30 miRNA families. Quantitative real-time reverse transcription PCR verified that the expressions of all 19 differentially expressed miRNAs were basically consistent with the sequencing results. Our study identified miRNAs that might participate in pollen development and provided the relationship between miRNAs and their target genes, which might form the theoretical basis for future research.
关键词: Chinese cabbage genetic male sterility pollen development miRNA high-throughput sequencing
基金:
论文图表:
引用
No.4688704114819314****
同行评议
勘误表
Identification of microRNAs related to the genetic male sterility of Chinese cabbage using high-throughput sequencing
评论
全部评论0/1000