Comparative transcriptome analysis of a female-sterile mutant (fsm) in Chinese cabbage (Brassica campestris ssp. pekinensis)

• 1、Department of Horticulture, Shenyang Agricultural University, 110866

Abstract：In this study, we identified the female-sterile mutant fsm in Chinese cabbage. This mutant, which exhibits stable inheritance, was derived from Chinese cabbage DH line 'FT' using a combination of isolated microspore culture and ethyl methanesulfonate (EMS) mutagenesis. Genetic analysis indicated that the phenotype of fsm is controlled by a single recessive nuclear gene. Morphological observations revealed significant differences between the floral organs of fsm and wild-type line 'FT'. Parts of the pistils of fsm are smaller and shorter than those of 'FT', especially the ovary, which may directly cause the female sterility of the mutant. Comparative transcriptome analysis of 'FT' and fsm using RNA-Seq revealed a total of 1,872 differentially expressed genes (DEGs) between 'FT' and fsm. Of these, a number of genes involved in ovule development were identified, such as PRETTY FEW SEEDS 2 (PFS2) and Temperature-Induced Lipocalin (TIL), which were upregulated in fsm vs. 'FT'. In addition, qRT-PCR analysis of the expression patterns of 18 DEGs confirmed the accuracy of the RNA-seq data. These results shed light on the molecular mechanisms underlying pistil development in Chinese cabbage.

keywords： Vegetable science Chinese cabbage Female sterility Transcriptome analysis RNA-Sequencing

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