多功能碳量子点介导microRNA细胞内示踪与转运的研究
首发时间:2016-05-23
摘要:MicroRNA ( miRNA )能降解靶基因的mRNA或者抑制靶基因的翻译,具有巨大的基础医学研究与临床治疗的潜能。然而,尚缺乏兼具胞内示踪与基因转运的多功能基因载体。本文利用一步水热法制备透明质酸-聚乙烯亚胺功能化碳量子点(HA-PEI functionalized carbon dots, HP-CDs)。通过透射电子显微镜(TEM)、傅里叶变换红外光谱(FITR)、荧光光谱(PL)、X射线衍射光谱(XRD)对其进行理化表征。结果表明,HP-CDs具有较为均一的粒径分布(~ 3.2 nm),丰富的亲水表面基团,稳定的荧光性能和较高的荧光量子产率(13.2%)。细胞实验表明,HP-CDs具有较好的生物相容性,基因转载能力以及介导miRNA-30b转染的能力。进入细胞内部的HP-CDs可发出明亮的荧光,可作为有效的示踪基因载体用于miRNA胞内的可视化研究。
关键词: 生物工程 透明质酸 聚乙烯亚胺 碳量子点 肿瘤靶向 基因转运
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Multifunctional carbon dots for microRNA delivery and real-time tracking
Abstract:MicroRNAs have great potential in preclinical medicine research and clinical treatment due to they can inhibit target genes expression by transcription level or protein level. However, there is lack of gene carriers which can be used in intracelluar tracking and gene transportation. Hyaluronic acid-polyethyleneimine functionalized carbon dots (HP-CDs) were prepared by one-step hydrothemal method. TEM, FITR, PL, XRD were used to characterize the physiochemical perperties of HP-CDs. These results demonstrated that HP-CDs possessed uniform size distribution (~ 3.2 nm), a large number of hydrophobic groups, stable fluorescence and high quantum yeild (13.2%). Meanwhile, HP-CDs with favorable biocompatibility, gene loading and transfection capacity can be served as a kind of effective tracking gene vector for visualization research of microRNA.
Keywords: bio -medicine;hyaluronic Acid;polyethylenimine;nitrogen-doped carbon dots;gene transportation
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