Effect of SIRT1 expression on the autophagy of chondrocytes in vitro

• 1、The fourth Affiliated hospital of Anhui medical university
• 2、The first Affiliated hospital of Anhui medical university

Abstract：[Objective] To study the effect of SIRT1 on the autophagy of normal and osteoarthritis chondrocytes. [Methods] SIRT1 agonist (resveratrol) and inhibitor sirtinol change chondrocytes endogenous SIRT1 activity, and acetylation activity detection reagent box verification SIRT1 activity of SIRT1; by immunohistochemistry to observe the effect of SIRT1 modulators of cartilage cell protein expression; Western blot detection of autophagy related protein beclin-1 expression; and the MDC staining and electron microscope observation of the morphological changes of chondrocytes autophagy. [Results] SIRT1 was mainly expressed in the nucleus SIRT1 in two groups of chondrocytes expression slightly different, more expression in the normal group, the cartilage cells, expression is low in the osteoarthritis group cells, when sirtinol 80nm role in chondrocytes of normal group 12 h, Beclin-1/GAPDH by former role of 0.92±0.14 reduced to 0.24±0.11; resveratrol 10μM at 12h after Beclin-1/GAPDH ratio decreased in osteoarthritis chondrocyte group, have statistical difference (P < 0.05). Detection of Beclin-1 by blot Western was consistent with the results of MDC staining. [Conclusion] SIRT1 is able to participate in the regulation of autophagy, SIRT1 may be a more important target in the treatment of osteoarthritis.

Keywords： Ostearthritis Autophagy Chondrocyte Aging SIRT1