交替残差三线性化算法结合三维荧光光谱应用于多色荧光基团的DNA序列同时检测
首发时间:2016-06-15
摘要:本文提出了一种新的二阶校正方法--交替残差三线性化(ART),用于解决多种靶序列同时定量检测时分子信标荧光光谱重叠的问题。分子信标探针技术目前被广泛应用于小分子、蛋白和核酸的分析检测中。但由于标记分子信标的荧光分子种类少、光谱带宽,严重限制了分子信标在多组分同时分析中的应用。与传统的二阶校正平行因子分析方法(PARAFAC)相比,ART无需预先估计组分数N,而是在收敛过程中自行确定,提高了分子信标检测的稳健性。为了规避荧光光谱中不符合三线性的瑞利散射干扰,本文在ART算法中结合采用了缺损数据重构(MDR)算法。数据分析结果显示,在三种分子信标荧光光谱重叠非常严重的情况下,ART分辨得到的解析光谱与真实光谱高度吻合,其定量结果与真实浓度基本一致,证实了ART的有效性。
关键词: 分析化学 分子信标 荧光光谱 缺损数据重构 交替残差三线性化
For information in English, please click here
Simultaneous determination of multicolor DNA sequences using excitation-emission matrix fluorescence coupled with alternating residuals trilinearinzation algorithm
Abstract:Molecular beacon (MB) probe technology has extensive applications in the analysis of small molecule, protein and nucleic acid. But the very few kinds of labeled fluorophores on MB and the wide bandwidth of their fluorescence both limit its application in multicomponent simultaneous detection. In order to improve the detection performance of the MB analysis, we provide a new second-order calibration method, alternating residuals trilinearization (ART) algorithm, which could solve the spectrum overlapping problem in multicomponent fluorescent MBs and simultaneously determine multiple targets. Compared to the conventional second-order calibration method PARAFAC, the number of components could be obtained automatically in the convergence process in ART algorithm, rather than be determined individually prior to the analysis. Besides, the missing data recovery algorithm is coupled with the ART method to avoid the impact of Rayleigh scattering signal interference. Though the excitation-emission spectra of the three MBs are heavily overlapped, it also can be resolved by the algorithms well. The satisfactory quantitative results and resolved spectra are obtained.
Keywords: Analytical chemistry Molecular beacon Fluorescence spectrum Missing data recovery Alternating residuals trilinearization
基金:
论文图表:
引用
No.4695732115354314****
同行评议
共计0人参与
勘误表
交替残差三线性化算法结合三维荧光光谱应用于多色荧光基团的DNA序列同时检测
评论
全部评论0/1000