Antitumor activity analysis of endophytic fungi isolated from antitumor medicinal plants

• 1、Department of Pathogenobiology, College of Basic Medical Sciences, Jilin University, Changchun 130021

Abstract：Object With increasing incidence and mortality, cancer is the leading cause of death and is a major public health problem. And there are some problems of clinical antitumor drugs. Endophytic fungi have variety natural bioactive products, which is a potential source of new antitumor drugs or new lead compounds. Methods Thirteen species of antitumor medicinal plants in Changbai Mountain were collected. Endophytic fungi were isolated and identified according to morphology. By MTT assay based on inhibition rate and IC50 values on Hela cells, endophytic fungi with a higher antitumor activity were selected, and the effects of endophytic fungal active ingredient on Hela cells were observed. Results There are 17 genera and 108 strains of endophytic fungi were isolated from antitumor medicinal plants with species diversity. The secondary metabolites of 4 isolates had better antitumor activity, with the inhibition rate on Hela cells ≥85%, and 3isolates with IC50 values≤50μg/mL. The effects of active ingredients of endophytic fungi FI 29 with the highest antitumor activity on Hela cells showed an obviously concentration and time dependent manner. Conclusions The endophytic fungi isolated antitumor medicinal plants have variety diversity. The endophytic fungi active ingredient had a higher inhibitory effect on tumor cells is excavated in this study, which is useful resources for the research of natural and effective antitumor drugs or new drug lead compounds.

Keywords： endophytic fungi medicinal plants metabolites antitumor activity