Ascobic acid-based carbon dots as carrier of miR-2861 to promote differentiation of osteoblast

• 1、Department of Pathology, School of Stomatology, Jilin University, Changchun 130021，
• 2、Department of Periodontics, School of Stomatology, Jilin University, Changchun 130021，
• 3、Department of Pediatric Dentistry, School of Stomatology, Jilin University, Changchun 130021，

Abstract：Using carbon dots as gene vector mediated miR-2861 to transfer into rBMSCs. To expore the effect of CD/miR-2861 on osteogenic differentiation in rBMSCs. The carbon dots were synthesized by condensing ascorbic acid and PEI through "one-step" microwave-assisted method. The mean particle size and zeta potential of carbon dots were 2.8 nm and 33 mV characterized by TEM and dynamic light scattering, respectively. Results from fluorescence spectra showed that emision of carbon dots was excitation-dependented. The carbon dots can be used in cell imaging, which was demonstrated by fluorescence microscope. Results from real-time PCR showed the carbon dots can promote the expression of osteogenic related gene. Meanwhile, fluorescence microscope showed that the carbon dots can mediate miR-2861 to transfect into rBMSCs. In the transfection experiments in vitro, the expression oteogenic related gene and the formation of calcium deposits in CD/miR-2861 transfected cells were enhanced, which was proved by real-time PCR and alizarin red staining, respectively. It turned out that carbon dots as gene vector can mediate miR-2861 to transfect into rBMSCs, and CD/miR-2861 can promote osteogenic differentiation of rBMSCs.

Keywords： carbon dots miR-2861 osteogenic differentiation