Specific cellular response in tumor bearing mice induced by FAP DNA immunization combined with zymosan

• 1、National Engineering Laboratory of AIDs vaccine, Jilin university

Abstract：Objective: To observe the anti-tumor immune responses elicited by FAP DNA vaccine immunization with zymosan in tumor-bearing BALB/c mice. Methods: BALB/c mice were inoculated intramuscularly with the vaccine plasmid encoding FAP with zymosan, at the dosage of 100μg plasmid and 2mg of zymosan in quadriceps muscle three times at two weeks interva1．Two weeks after the last injection，2 × 104 4T1 tumor cells was injected subcutaneously into back of mice Tumor growth was measured every two days with a caliper, and the tumor volume was calculated until 22 days. After sacrifice, the spleens were collected for assays of IFN-γ secretion and the proliferation activity. Results: Enhanced specific IFN-γresponse was detected through ELISPOT in DNA+zymosan immunized mice(P<0.05) and DNA combined with zymosan improved lymphocyte proliferation by 10%. This strategy inhibited the tumor growth significantly (P<0.05). Conclusions: The DNA vaccine CpVR-FAP combined with zymosan can induce in vivo the cell mediated specific immune responses and anti-tumor effects in tumor-bearing mice, which lays a certain foundation for development of prophylactic and therapeutic tumor gene vaccines．

Keywords： Fibroblast activation protein DNA vaccine IFN-γ immune response