LncRNA-ANCR沉默对骨肉瘤细胞凋亡的影响
首发时间:2016-07-13
摘要:目的 探讨ANCR与骨肉瘤细胞凋亡的关系。方法 通过流式细胞仪检测不同组别的U2OS的细胞凋亡情况,并模拟临床用药,分组联合应用顺铂干预U2OS(shCon-阴性对照组,shANCR-ANCR干扰组,Cisplatin-顺铂组,shANCR+Cisplatin- shANCR+顺铂组),通过流式细胞仪检测4组细胞凋亡比例。结果 shANCR组骨肉瘤细胞凋亡比例为12.6%,shCon组为5.7%,Con组为4.6%,shANCR组细胞凋亡比例显著高于其余2组,差异有统计学意义(P<0.05);而空白对照组与阴性对照组间的凋亡比例无明显差异(P>0.05)。在联合顺铂干预后发现顺铂组U2OS凋亡比例为9.7%,shANCR组为11.9%,shCon组为4.6%,而shANCR+顺铂组为23.7%。shANCR组及顺铂组的凋亡比例均高于阴性对照组(P<0.01),但二者之间没有统计学差异(P>0.05);shANCR+顺铂组的诱导骨肉瘤细胞凋亡比例为23.7%,明显高于其余各组(P<0.01)。结论 ANCR敲减后能够增加U2OS细胞的凋亡比例,凋亡的启动和执行能力提高,作用与顺铂体外治疗作用相近;而且ANCR敲减后与顺铂协同作用可以增加骨肉瘤细胞的凋亡,从而增强对化疗的敏感性。?????
关键词: 骨肉瘤 ANCR 细胞凋亡?????
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Effects of LncRNA ANCR inactivation on osteosarcoma apoptosis
Abstract:Objective To explore the possible molecular mechanism of osteosarcoma cells apoptois through inactivation of LncRNA ANCR. Methods To explore the apoptosis level of U2OS before/after cisplatin treatment. To investigate the molecular mechanism of ANCR for osteosarcoma cell line with the treatment of cisplatin or/and shANCR. Results The percent of apoptotic osteosarcoma were 12.6%,5.7%and 4.6% in shANCR group, shCon group and Con group respectively,and apoptosis in ANCR knockdown cells were increased compared with the other series of cells(P<0.01),which were not different statistically(P>0.05).And the percent of apoptosis in the shCon cells that were treated by cisplatin was 9.7%,which were not different statistically with shANCR cells(11.9%). However, the addition of cisplatin significantly increased apoptosis in ANCR knockdown cells, and the percent was 23.7%.Conclusions ANCR knock down could increase the apoptosis of osteosarcoma cells, and the combination of cisplatin treatment and ANCR inhibition could further promote apoptosis of osteosarcoma and increase the sensibility of osteosarcoma for cisplatin.?????
Keywords: Osteosarcoma Anti-differentiationnon-coding RNA Apoptosis
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