Bioinformatical analysis of Defensin in Musca domestica, from its transcriptome database

• 1、College of Life Sciences,Hebei University, Baoding,071002
• 2、College of Life Sciences, Hebei University, Baoding, 071002

Abstract：【Aim】 Antimicrobial peptides are a result of the organisms category by specific genes encoding small molecule polypeptide, is a important component of the biology immune system. Through the transcriptome level housefly (Musca domestica) antibacterial peptide defense screening of gene families and bioinformatics analysis, for the further study of housefly defensive element to lay a good foundation in the immune system function.【Methods】Based on the transcriptome data, through bioinformatics methods for housefly defense gene screening and analysis of system evolution and structure prediction.【Results】 in housefly transcription group identified five defense protein gene, its coded protein contains 40-73 amino acids, about 4-7.82 kD molecular weight, isoelectric point of 4.72-8.96, and predict their secondary and tertiary structure.【Conclusion】 The analysis show that the use of housefly transcriptome data screening of defense genes tend to be conservative in the sequence and protein, the results in order to further clarify the family provides important theoretical basis for immune function.

Keywords： Musca domestica；Defensin；Bioinformatics；Transcriptome