Phosphoproteomic Analysis of Arabidopsis Pollen Development

• 1、College of Life Science, China Agricultural University， Beijing,100193

Abstract：Pollen tube as a carrier to deliver the sperm to the ovule during the process of sexual plant reproduction in higher plants. Pollen tube growth is highly polarized and has become a relatively well-characterized model system in which to study cell-cell recognition, intra- and intercellular signaling in higher plants. To investigate the roles of the protein phosphorylation in the growth of pollen tube of Picea meyeri Rehd. et Wils., a quantitative approach was used to analyze the protein phosphorylation dynamic changes during pollen development. A total of 44 phosphoproteins were reproducibly identified in this study, among the identified proteins, in case of 25 proteins are increased on the phosphorylation level while 19 proteins are decreased after pollen germination.. These proteins were grouped into distinct functional categories including proteins involved in signaling transduction, membrane trafficking, cytoskeleton and carbohydrate metabolism. These data indicated that energy metabolism was increased an active level, furthermore, protein phosphorylation play a crucial role in the regulation of membrane trafficking pathways in growing pollen tubes.

Keywords： Pollen Protein phosphorylation Membrane trafficking Cytoskeleton?????