牛繁缕对苯磺隆的代谢抗性
首发时间:2016-12-13
摘要:为探讨牛繁缕对苯磺隆的代谢抗性机制,测定了敏感、抗性牛繁缕代谢酶谷胱甘肽-S-转移酶(GSTs)、细胞色素P450单加氧酶(P450s)活性差异。试验结果表明,未施药处理组抗性和敏感牛繁缕的GSTs活力随时间有一定波动,但差异不大。苯磺隆茎叶喷雾处理后,所有种群的GSTs活性均呈现出先升高后下降,最终趋于平稳。抗性JS-16种群的GSTs活性上升速度较快,第2天达到最高;敏感种群JS24和抗性种群JS17的GSTs活力上升较之JS16较为缓慢,在第3天达到最高;药剂处理后,抗性JS-17种群的GSTs活性显著低于敏感种群,而JS-16种群的GSTs活力高于敏感种群。马拉硫磷与苯磺隆共同处理牛繁缕与单独使用苯磺隆相比,抗性与敏感种群的对苯磺隆敏感性均有所增强:敏感种群JS-24和抗性种群JS-16的GR50下降倍数分别为2.38、2.55;抗性种群JS-17的下降倍数最高为4.13。本研究表明代谢酶GSTs和P450s活性增强可能是牛繁缕种群JS16和JS17对苯磺隆产生高水平抗性的重要原因之一。
关键词: 农药学 牛繁缕; 苯磺隆; 抗药性; 代谢酶。
For information in English, please click here
The enhanceed GSTs and P450s activity in tribenuron-methyl-resistant
Abstract:To investigate the metabolic resistance mechanism to tribenuron in water chickweed, this study detected the cytochrome P450 monooxygenase (P450s) and glutathione-S-transferase (GST) activity assay of sensitive and resistant populations. Results showed that P450 inhibitor malathion increased tribenuron sensitivity in all populations. The decrease in the amount of herbicide dose that causes 50% growth reduction (GR50) for the sensitive (S) population JS24 and the resistant (R) populations JS16 and JS17 were 2.38-, 2.55-, and 4.13-fold, respectively. However, the GR50 values for the R populations were still much higher than those of the S population. This observation indicates that P450-mediated enhanced metabolismis one mechanism for resistance in water chickweed. The GST activity could be induced by tribenuron for all tested populations. In particular, the GST activity of JS16 is inherently greater and is more rapidly induced than that of JS17 or JS24. This study establishes that either one or at least two resistance mechanisms are involved in herbicide resistance in water chickweed. Moreover, these mechanisms might contribute to the different levels of resistance to tribenuron among water chickweed populations.
Keywords: Pesticide Water chickweed (Myosoton aquaticum L.) tribenuron-methyl herbicide resistance cytochrome P450 monooxygenase (P450s) glutathione-S-transferase (GST).
论文图表:
引用
No.4712812117285414****
同行评议
共计0人参与
勘误表
牛繁缕对苯磺隆的代谢抗性
评论
全部评论0/1000