Immunization difference of total alkaloids extracted from original and processed Stemona Tuberosa on asthma rat

• 1、(College of Pharmacy, Liaoning University of Traditional Chinese Medicine ( TCM )

Abstract：Immunization difference of total alkaloids extracted from crude and processed Stemona tuberose was studied based on OVA-induced asthma mice model, and chemical difference was analyzed by UPLC-MS to explore the material foundation for pharmacological change. Mice were orally administered with different concentration of total alkaloids, and the cytokine levels from T-cells in splenocytes were determined by ELISA. RT-PCR was carried out to describe the ratio of T-bet /GATA-3. The results showed a significant decrease in IFN-γ, IL-2, IL-13 levels and an increase in IL-4 and IL-5 levels in the model group. Compared with crude extract, middle and high group of processed samples significantly increased the levels of INF-? （P<0.05）and all three groups decreased that of IL-5（P<0.05）. The PCR test results showed processed samples increased the ratio of T-bet /GATA-3（P<0.05）. This study indicated that total alkaloids extract from crude and processed Stemona Tuberosa could treat asthma by regulating Th2/Th1 imbalance and the processed one had a more significant effect, which might be caused by the change in the ratio of alkaloids after being processed This paper lays the foundation for further revealing the processing principle of Stemona Tuberosa and provides the experimental support for further development of antitussive medicine of Stemona Tuberosa.

Keywords： processing total alkaloids extracted from crude and processed Stemona tuberose asthma cytokine GATA-3 T-Bet