The Expression Pattern and Splicing Variant Analysis of Lysine Methyltransferase Kmt5a in Pig Testicles

• 1、College of Animal Science and Technology of Northwest A&F University, Yangling, Shaanxi, China Postcode: 712100

Abstract：Lysine methyltransferase 5A (KMT5A) plays an important function in histone methylation, cell division, apoptosis and other physiological activities. This study interpreted the expression pattern of Kmt5a gene in pig testicles by cloning two different splicing variants Kmt5a-X1 and Kmt5a-X2, detecting the relative expression quantity change of Kmt5a gene in the development process of pig testicles, analysing of the structural differences between the splicing variants. The results showed that the relative expression quantity of Kmt5a in 7-day-old sertoli cells were significantly higher than which in interstitial cells and gonocytes of testicles. The global level of Kmt5a relative expression quantity in 5-month-old testicles was significantly higher than those in 7-day-old and 2-month-old. The analysis showed that protein KMT5A-X1 was lack of PIP (PCNA-interacting motifs) box1 in the N-terminal compared with KMT5A-X2. The serine, a potential phosphorylation sites of Cdk1/Cyc B complex, was located at amino acid residues 2 of KMT5A-X1. Presumably its special position could affect the ability to combine with Cdk1/Cyc B complex.

Keywords： testicle Kmt5a splicing variant protein structure