The effects on reproductive toxicity of scallop Chlamysfarreri exposed to benzo[a]pyrene

• 1、The Ocean University of China, Fisheries College, Qingdao, 266003

Abstract：The study investigated the reproductive toxicity effects of benzo pyrene (BaP) on scallop Chlamys farreri, and the results showed that BaP could significantly interfere the levels of sex hormone including estradiol (E2) and testosterone (T) in the gonad of Chlamys farreri (P < 0.05). The two hormones decreased significantly in ovarian, and it showed dose-effect relationship, as well as BaP could significantly inhibit the content of E2 in testis while the levels of T increased. Comparing with the control group, the levels of GSI in 0.5 and 2.5μg/L BaP treatment groups decreased significantly, while the 0.1μg/L BaP treatment group had no significant change. Different concentrations of BaP caused significant DNA damage, protein carbonylation and lipid oxidation degree increased in gonads. The 0.5, 2.5μg/L of BaP exposed on Chlamys Scallop in gonad showed significant DNA damage (P<0.05), and the degree of damage increased as well as the exposed time. The protein carbonyl content increased first and then decreased and increased finally in BaP treatment group of testis and ovary, while at the end of experiment the contents of BaP treatment groups were significantly higher than the control group. MDA content of each sex increased gradually, and the contents of 2.5μg/L BaP group at 15d were significantly higher than those in control group. BaP could significantly affect the gonadal development in Chlamys Scallop, including anti the proliferation of germ cells and make them deformation. In addition, BaP could damage the structure of gonadal tissue in the follicular wall. The results showed that BaP had caused the endocrine disrupting effects and damage to the tissues of Chlamys Scallop, thus it caused significant toxicity to the reproduction.

Keywords： aquatic biology；BaP Chlamys farreri endocrine disruption gonadal damage microstructure