1,25（OH）2D3对谷氨酸诱导HT-22细胞损伤保护作用研究

马书杰; 王建荣; 贺金奖; 李冰燕; 张增利

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Protective Effect of 1, 25(OH) 2D3 on Glutamate-Induced Cytotoxicity in HT-22 Cell

首发时间：2017-02-10

Ma Shujie ¹
Ma Shujie(1991-),Female,Graduation,nourishment and chronic disease
Wang Jianrong ² He Jinjiang ³ Li Bingyan ⁴ Zhang Zengli ¹
Zhang Zengli(1967-), Male, Professor,Nourishment and chronic disease

1、Department of Labor Hygiene and Environmental Health, School of Public Health of Soochow University,Suzhou 215123
2、The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830011
3、Shanghai Entry-Exit Inspection and Quarantine Bureau Hongkou Office, Shanghai 200135
4、Department of Nutrition and Food Hygiene, School of Public Health, Soochow Uniiversity, Suzhou 215123

Abstract：1,25(OH)2D3 deficiency may be associated with affective disorders, Parkinson's disease, Alzheimer's disease and cognitive abilities. To explore the protective effects of 1,25(OH)2D3 against glutamate-induced damage in a mouse-derived hippocampal HT-22 cells and underlying potential mechanism. The cell survival rate was determined with Cell Counting Kit-8. Cell cycle, apoptosis and reactive oxidative species (ROS) release were detected by FACs technology. Results showed that pretreatment 1,25(OH)2D3 effectively increase HT-22 cell viability up on glutamate exposure. This might be associated with the fact that 1,25(OH)2D3 significantly reduced the percentage of G0/G1 circles (G0/G1%) (p<0.01)，increased the proportion of S phase (S%) and G2/M phase (G2/M%) cells (p<0.01). Moreover, apoptosis in HT-22 cells showed the late cell apoptosis rate was decreased (p<0.05) in glutamate group incubated with 1,25(OH)2D3 (i.e. combination group) compared with the glutamate only group. There was an inverse association between the concentration of 1,25(OH)2D3 and apoptosis rate. Meanwhile, the ROS release was elevated in the combination group (p<0.05). Our study indicates that 1,25(OH)2D3 might adversely affect glutamate-induced apoptosis in the HT-22 cells，this might be owing to the fact that 1,25(OH)2D3 could increase cell proliferation and decrease late apoptosis rate. 1,25(OH)2D3 might be a promising therapeutic agent for the treatment of depression.

keywords： 1, 25-dihydroxyvitamin D3 Glutamate HT-22 cells Neurotoxicity

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1,25（OH）2D3对谷氨酸诱导HT-22细胞损伤保护作用研究

马书杰 ¹
Ma Shujie(1991-),Female,Graduation,nourishment and chronic disease
王建荣 ² 贺金奖 ³ 李冰燕 ⁴ 张增利 ¹
Zhang Zengli(1967-), Male, Professor,Nourishment and chronic disease

1、苏州大学公共卫生学院劳动卫生与环境卫生学
2、新疆医科大学第五附属医院
3、上海出入境检验检疫局虹口办事处
4、苏州大学公共卫生学院营养与食品卫生学

摘要：目的：探讨1,25（OH）2D3 对谷氨酸（Glu）诱导的HT-22细胞损伤的保护作用，及其潜在的可能机制。方法：本研究细胞分为对照组、1,25（OH）2D3组、谷氨酸组与联合作用组。采用CCK-8测定细胞存活率，用流式细胞仪进行细胞周期、细胞凋亡和细胞活性氧（reactive oxidative species，ROS）的检测。结果：1,25（OH）2D3 预处理HT-22细胞可以有效提高谷氨酸作用后的细胞存活率。细胞周期显示1,25（OH）2D3 可以明显减少谷氨酸作用后的G0/G1期细胞比例，增加S期与G2/M期细胞比例。相对于谷氨酸组，联合作用组细胞晚期凋亡率均有下降，随1,25（OH）2D3 浓度增加凋亡率逐步降低。相对于对照组，各处理组均可以使细胞ROS释放增多（p<0.05）。结论：1,25（OH）2D3 可以拮抗谷氨酸诱导HT-22细胞产生的细胞死亡。这个过程可能与细胞增殖增多、晚期凋亡率下降有关。

关键词： 1,25-二羟基维生素D3 谷氨酸 HT-22细胞神经毒性

基金：

1. National Natural Science Foundation（81372981, 81372979）

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Ma Shujie,Wang Jianrong,He Jinjiang,et al. Protective Effect of 1, 25(OH) 2D3 on Glutamate-Induced Cytotoxicity in HT-22 Cell[EB/OL]. Beijing:Sciencepaper Online[2017-02-10]. https://www.paper.edu.cn/releasepaper/content/201702-57.

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论文编号	201702-57
论文题目	1,25（OH）2D3对谷氨酸诱导HT-22细胞损伤保护作用研究
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