四氢生物碟呤与产油真菌高山被孢霉的脂质合成相关性研究
首发时间:2017-03-10
摘要:GTP环式水解酶(GTPCH)是四氢生物蝶呤(BH4)从头合成途径的限速酶。本文通过构建GTPCH基因的过表达重组菌株,对过表达重组菌株中的BH4含量,NADPH含量及脂肪酸含量进行分析,来研究BH4与高山被孢霉脂质合成的关系。过表达GTPCH基因后,与野生型高山被孢霉相比,重组菌株中BH4含量增加了7倍,NADPH含量增加了2倍左右,总脂肪酸增加了37%。结果表明,四氢生物碟呤与产油真菌高山被孢霉的脂质合成存在相关性,BH4通过提高细胞内还原力的水平来调控脂质合成,这可能是BH4对高山被孢霉脂质合成的作用机制。
关键词: 高山被孢霉; 四氢生物蝶呤; GTP环式水解酶; NADPH 脂质合成
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Study on the correlation of tetrahydrobiopterin to lipogenesis in oleaginous fungus Mortierella alpina
Abstract:GTP cyclohydrolase I (GTPCH) is the first and rate-limiting enzyme for biosynthesis of tetrahydrobiopterin (BH4). GTPCH was overexpressed to analyze the BH4content, NADPH level and fatty acid content in GTPCH overexpression strain (MA-GTPCH), and investigate the correlation of tetrahydrobiopterin to lipogenesis in Mortierella alpina. The generation of GTPCH transgenic M. alpina strains led to a 7-fold increase in BH4 level, increased NADPH levels by 2-fold, increased lipid levels by 37% in MA-GTPCH compared with wild type M. alpina. These results indicate that BH4 plays an important role in lipogenesis in Mortierella alpina, BH4 can augment lipid production by enhancing NADPH level, which may be its mechanism of effect on lipogenesis in Mortierella alpina.
Keywords: Mortierella alpina tetrahydrobiopterin GTP cyclohydrolase I NADPH lipogenesis
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