Novel Function of ybtS gene to modulate metabolism in Pathogenic Escherichia Coli

• 1、School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331
• 2、Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240

Abstract：Pathogenic escherichia coli UTI89 strains could lead to severe urinary tract infections (UTI) which affected the women's health, meanwhile, the virulence factor, yersiniabactin, as one siderophore encoded by highly pathogenic islands (HPI), which directly contributed to the growth, reproduction and pathogenicity of bacteria. Previous research reported that the function of ybtS geng on HPI often produced the salicylic acid, a key substance of yersiniabactin. Here, we attempt to dissect more new functions about ybtS gene at metabolic level. The strategy by integrating genetics and metabolomics that based on the LC-MS/MS platform was applied for capturing the differential metabolism of the pellets and supernatants of UTI89 strain group, UTI89ΔybtS mutant strain group and UTI89ΔybtS+SA with salicylic acid complementation groups. 114 significant metabolites was discovered and identified for the first instance, associated metabolic pathwayswere annotated as well. At last, our data revealed that the primary new-function of ybtS was characterized by indirectly regulating the transmembrane transport of yersiniabactin beyound guiding the biosynthesis of salicylic acid, via modulating the targeted metabolism. Altogether, thisnew function of the ybtS gene is supposed to provide novelinsights into pathogenic characteristics and virulent formation of UPEC E. coli, as well as, offernew foundation for the new antibiotics development based on the modified siderophores.

Keywords： Metabolomics, Urinary Tract Infection,UropathogenicEscherichia Coli,Siderophore