ALDH2调控线粒体分裂的分子机制研究
首发时间:2017-04-27
摘要:乙醛脱氢酶2(ALDH2)是位于线粒体基质内的酶,在心脏缺血-再灌注过程中,具有重要的心肌保护作用。为了研究ALDH2对线粒体形态的调控,我们选用大鼠心肌细胞H9C2作为细胞模型,构建ALDH2过表达的H9C2细胞系,给予细胞缺氧-复氧处理;并在荧光显微镜下观察线粒体的形态变化;检测腺苷酸激活蛋白激酶(AMPK)及线粒体分裂调控蛋白Drp1的表达情况。本实验发现过表达ALDH2可影响线粒体的形态,并导致AMPK的表达水平升高、AMPK磷酸化水平下降、Dpr1 Ser616的磷酸化水平降低。以上结果表明ALDH2通过AMPK-Drp1信号通路对线粒体形态的维持发挥调节作用。
关键词: ALDH2 缺氧-复氧 线粒体分裂 AMPK Drp1
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Molecular mechanisms of ALDH2 regulates mitochondrial fission
Abstract:ALDH2 (Acetaldehyde dehydrogenase 2) which located in mitochondrial matrix, plays a myocardiac protective role in hypoxia-reoxygennation. Here, we emphasize to explore the roles of ALDH2 in the morphology of mitochondria. We generated ALDH2 overexpression H9C2 cell lines by adenoviral infection, and we detect the effect of ALDH2 on the morphology of mitochondria after hypoxia-reoxygennation through fluorescence microscope and detect the expression level of AMPK and Drp1. We found that ALDH2 overexpression H9C2 cells exhibit normal morphology of mitochondia after hypoxia-reoxygennation. Overexpression of ALDH2 affects the AMPK expression level, AMPK phosphorylation level and Drp1 phosphorylation level. These results indicating the important roles of ALDH2 in the maintenance of mitochondrial morphology.
Keywords: ALDH2 hypoxia-reoxygennation mitochondria fission AMPK Drp1
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No.4728280119544714****
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