A new transgenic mouse model of spinal cord injury
首发时间:2017-04-11
Abstract:Objective: To explore a new model for the study of spinal cord injury, and to prove the possibility of this model. Methods: The Thy1( thymus cell antigen 1, theta)-YFPH transgenic mice and DiI tracing were employed to observe the distribution of corticospinal tract (CST). And lateral spinal cord hemisection injury model was made using the Thy1-YFPH mice. Results: Pyramidal neurons in cerebral cortex layer Ⅴ was specifically labeled and projected to pyramidal decussation in the medulla. In the spinal cord, the YFP labeled projections were distributed at the same locations as dorsal CST (dCST), dorsolateral CST (dlCST) and ventral CST (vCST). YFP positive axons were mostly colocalized with DiI traced dCST both in the spinal cord of uninjured Thy1-YFPH mice. Conclusion: The Thy1-YFPH mice could label the CST in the mouse spinal cord, and could be used as an easy and convenient model to monitor the degeneration, regeneration and remodeling of CST axons after spinal cord injury.
keywords: spinal cord injury CST Thy1-YFPH transgenic mice
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一种新的研究脊髓损伤的转基因小鼠模型
摘要:目的:建立一种新的研究脊髓损伤的模型,并证明该模型的可行性。方法:Thy1( thymus cell antigen 1, theta)-YFPH转基因小鼠建立脊髓半横断损伤模型。通过Thy1-YFPH转基因小鼠特异性标记和DiI示踪标记法,观察皮质脊髓束的分布。结果:位于脑皮层V层的锥体神经元被特异性标记,并且这些特异性标记一直延伸分布道锥体交叉。在脊髓损伤的Thy1-YFPH转基因小鼠中,黄色荧光蛋白分布在皮质脊髓背侧束,背外侧束,以及腹侧束。在脊髓未损伤的Thy1-YFPH转基因小鼠中,黄色荧光蛋白标记的神经元大多数位于DiI标记的皮质脊髓背侧束。结论:Thy1-YFPH转基因小鼠能够标记脊髓损伤模型中皮质脊髓束,该转基因小鼠模型可直接用于观察脊髓损伤后的皮质脊髓束的再生,重生,以及重塑。
关键词: 神经生物学 脊髓损伤 皮质脊髓束 Thy1-YFPH转基因小鼠
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