Transcriptome sequencing and phylogenetic analysis of four species of luminescent beetles
首发时间:2017-05-02
Abstract:The evolution of bioluminescence has prompted scientific attention to illuminate phylogenetic relationships of luminescent beetles. However, genomic resources are virtually lacking in rhagophthalmids (Rhagophthalmidae) and their related firefly beetles lampyrids (Lampyridae). Here, we employed the Illumina Hiseq 2000 platform and sequenced the whole-body transcriptomes of the four luminescent beetles: one rhagophthalmid (Rhagophthalmus sp.) and three fireflies (Asymmetricata circumdata, Aquatica ficta, and Pyrocoelia pectoralis). We obtained 55.4, 43.4, 38.6, and 36.7 million clean reads for the four species, respectively. All reads were assembled into contigs from which unigenes were derived. All unigenes were annotated by publicly available databases, and a total of 4325 orthologous genes were identified. Using multiple phylogenetic approaches, our transcriptome data confirmed the distinctiveness of Rhagophthalmidae from Lampyridae. Together, this study is the first report of whole transcriptome sequencing data in Rhagophthalmidae and Lampyridae species, representing a valuable genomic resource for studying the origin and evolution of some remarkable traits in these beetles such as bioluminescence. Moreover, our transcriptome data provide useful phylogenetic information that could be of importance in future studies of phylogenetic inference.
keywords: Rhagophthalmidae, Lampyridae, Phylogenomics, Transcriptome
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四种发光甲虫的转录组测序和系统发育分析
摘要:生物发光的起源和演化具有重要的科学意义和应用潜能,因此,发光甲虫的系统发育研究引起了很多学者的关注。然而,发光甲虫的基因组数据非常稀少,限制了其系统发育研究。本文利用二代测序技术,对4种发光甲虫(1种雌光萤、3种萤火虫)进行了全身转录组测序;对转录组进行组装和注释后,共鉴定出4325个严格的直系同源基因,可用于后续的系统发育重建。使用最大似然法和贝叶斯推断等方法重建系统发育关系,证实了雌光萤与萤科的萤火虫具有显著不同的系统位置,支持将雌光萤独立成一个科。总之,本研究第一次报道了雌光萤科和萤科昆虫的转录组数据,为研究发光甲虫的起源和演化提供了重要的基因组资源,也证实了这些转录组数据可以为将来的系统发育研究提供支撑。
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No.4727609119524514****
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