OsUreG基因突变对水稻生长的影响
首发时间:2017-05-16
摘要:细胞中脲酶活性的存在使得尿素能被水解为植物直接可利用的铵态氮。根据现有报道,脲酶的活性需要金属镍离子的活化及三种辅助蛋白UreD、UreG及UreF的协同作用。为了认识UreG基因功能对水稻生长、脲酶活性及氮代谢的影响,本文对水稻Osureg突变体株进行了不同氮源处理的生长表型及酶活的分析,发现与其相应野生型相比突变体表现出生长受到抑制而个体矮小,其株高、根长、地上部及地下部鲜重均低于野生型;同时也检测出其脲酶活性显著低于野生型。基于RT-PCR的基因表达分析,初步发现突变体中与脲酶活性相关的基因OsUrease和OsUreD的表达较野生型下调,而精氨酸酶基因OsArginase的表达量则无明显的差异。综上所述,本研究结果表明OsureG编码的脲酶辅助蛋白UreG对维持水稻脲酶活性、植物氮代谢以及生长发育等可能具有重要的分子生理学意义。
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Effect of OsUreG mutation on rice growth
Abstract:The existence of urease in the cytoplasm makes possible to hydrolyze urea into ammonium that can be directly utilized by the plant. It has been reported that the activity of urease requires nickel ion activation and a co-ordination of three accessory proteins i.e. UreD、UreG and UreF. To prelimiarily understand the effect of a function of UreG gene on rice growth, urease activity and nitrogen metabolism, in this work we examnined plant growth and enzyme activity of rice Osureg mutant cultivated under different nitrogen treatments. Our data showed that the mutant exhibited an obviuosly reduced growth phenotype with characteristics of a smaller size, shorter height and root length, lower fresh weight of shoot and rootas well assiganificantly lower urease activity, as compared to its corresponding wild type plant. By using RT-PCR, we detected an expression down-regulation of urease activity-related gene OsUrease and OsUreD in the mutant compared to that in the wild type, but no much differenence in the expression of arginase gene OsArginase between the mutant and wild type. In summary,OsUreG-coding protein UreG would play critical molecular physiological roles in the maintenance of plant urease activity, nitrogen metabolism as well as plant growth and development.
Keywords: Plant nutrition rice urea urease activity OsUreG
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