纤维母细胞高表达VEGF-C在头颈部肿瘤侵袭转移中的作用
首发时间:2017-05-17
摘要:目的 研究纤维母细胞中血管内皮生长因子C(vascular endothelial growth factor-C,VEGF-C)高表达与头颈部鳞癌肿瘤的生长与转移的关系。 方法 将带Luciferase基因的质粒转染到头颈部肿瘤细胞;带VEGF-C的质粒转染到成纤维3T3细胞,通过体内外实验,探索VEGF-C在头颈部鳞癌肿瘤的生长与转移中发挥的作用及其可能机制 结果 Western blot与ELISA实验共同证明VEGF-C基因已成功转染到上述头颈部肿瘤细胞中。细胞划痕实验验证了VEGF-C能够增强头颈部肿瘤细胞的侵袭、转移能力。体内成瘤实验观察到了两组大鼠体内肿瘤大小存在显著性差别,处死取出肿瘤后,肿瘤体积的差异性在统计学上具有显著性(P<0.05) 。 结论 VEGF-C是通过作用于肿瘤相关性纤维母细胞,在头颈部肿瘤的早期转移中起主导作用。
关键词: 头颈部鳞状上皮细胞癌 纤维母细胞 血管内皮生长因子-C
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The rold of high expression VEGF-C of fibroblasts in head and neck squamous cell
Abstract:Objective To study the relationship between the high expression of vascular endothelial growth factor C (VEGF-C) in fibroblasts and the growth and metastasis of head and neck squamous cell carcinoma. Methods We transfected plasmid containing Luciferase gene into head and neck cancer cells;We transfected plasmid containing VEGF-C into 3T3 fibroblasts,through experiments in vivo and in vitro to explore the role of VEGF-C in the growth and metastasis of head and neck squamous cell carcinoma and its possible mechanism. Results Western blot and ELISA experiments showed that VEGF-C gene was successfully transfected into the fibroblasts cells. There were significant differences in tumor size between the two groups of rats in vivo. The difference of tumor volume was statistically significant after the tumor was removed. (P < 0.05) Conclusions VEGF-C plays an leading role in the early metastasis of head and neck tumors by acting on cancer- associated fibroblasts.
Keywords: Head and neck squamous cell carcinoma fibroblast;vascular endothelial growth factor
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