从川芎苓子中筛选根腐病拮抗性内生真菌的研究
首发时间:2017-05-17
摘要:目的:从川芎苓子中筛查根腐病拮抗性内生功能真菌,以期为川芎根腐病的生物防治提供更有效的微生物资源。方法:利用组织块贴片法进行川芎内生真菌的分离,并以平板划线法进行菌种的纯化;通过形态鉴别法及分子鉴别法进行菌种的鉴别;再以4种川芎根腐病原菌为指示菌,采用平板对峙法进行川芎根腐病拮抗菌株地筛选。结果:从川芎苓子中共分离纯化了38株内生真菌,其中3株内生真菌表现出对川芎根腐病病原菌的拮抗作用。结论:针对川芎根腐病,川芎苓子中自带一定的"抵抗力"。
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Screening of Antagonistic Endophytic Fungi to Root Rot of Ligusticum chuanxiong Hort.
Abstract:The purpose of this study was to screen antagonistic endophytic fungi to the Root Rot form the Lingzi-nodes of Ligusticum chuanxiong hort.. The tissue block method was used for the isolation of endophytic fungi from the Lingzi of L. Chuanxiong. Purification of fungi by plate streak. Identification of fungi by morphological identification and molecular identification. The 4 strains of root rot pathogen of L. chuanxiong were used as indicator bacteria, and screening of antagonistic strains against Root Rot of L. chuanxiong by plate confrontation method. As a result, 38 strains of endophytic fungi were isolated and purified from the Lingzi of L. Chuanxiong. 3 of them showed antagonistic effects on Root Rot Pathogen. The results showed that the Lingzi of L. chuanxiong has some power of immunity against root rot.
Keywords: kTraditional Chinese Medicine Ligusticum chuanxiong hort. endophytic fungi Root Rot biocontrol bacteria
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No.4733857119912414****
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