家蚕BmPDCD2基因功能研究
首发时间:2017-05-19
摘要:细胞程序性死亡PCD(programmedcelldeath)是一种正常的生命现象,与之相关的蛋白参与细胞增殖、分化、凋亡等多个过程。本研究通过定量PCR的方法检测了家蚕PDCD2基因BmPDCD2在各组织中的表达情况。利用双链RNA(double-strand RNA, dsRNA)干扰和过表达的手段在家蚕BmN4细胞中分别上调和下调BmPDCD2,通过BrdU和TUNEL的方法探究了其在家蚕BmN4细胞中的功能。结果表明BmPDCD2基因在各组织中均有表达,且参与了细胞增殖与凋亡的调控,为进一步研究该基因在家蚕变态发育中的作用奠定了基础。
关键词: 细胞生物学; 家蚕; 增殖; 凋亡
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Function Exploration of BmPDCD2 in Bombyx mori
Abstract:Programmed cell death (PCD) is a normal life phenomenon, and the associated protein is involved in cell proliferation, differentiation, apoptosis and other processes. In this study, the expression of PDCD2 gene in silkworm was detected by quantitative PCR. BmPDCD2 was up-regulated and down-regulated in BmN4 cells by double-stranded RNA interference and overexpression. BrdU and TUNEL assays were used to explore its function in silkworm BmN4 cells. The results showed that the BmPDCD2 was expressed in all tissues, and it was involved in the regulation of cell proliferation and apoptosis, which laid a foundation for the further study of the role of this gene in the metamorphosis of silkworm.
Keywords: cell biology Silkworm, Bombyx mori cell proliferation apoptosis
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No.4734554120223214****
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