植物乳杆菌ZJ316的酵母凝集性及自身表面特性的研究
首发时间:2017-05-23
摘要:乳酸菌是人和动物肠道内的优势微生物菌群,益生性的乳酸菌在机体内有调节人和动物体微生态平衡,抑制并杀死致病菌,增强机体免疫力的作用。黏附是乳酸菌定植于宿主肠道发挥各种生理作用的前提。研究乳酸菌的黏附机制对于微生态制剂的开发应用具有重要的研究意义。本研究以本实验室保存的从婴儿粪便中筛选得到的一株产细菌素的植物乳杆菌(Lactobacillus plantarum ZJ316)为研究对象,用MATH的方法测定了三株乳酸菌(植物乳杆菌ZJ316、植物乳杆菌ZJ22、屎肠球菌ZJ19)的疏水性、表面电荷和自聚合能力等表面性质,并且进行了酵母凝集实验测定乳酸菌的糖类专一性,从而为更好的去研究乳酸菌的黏附机制打下了良好的基础。结果显示,植物乳杆菌ZJ316的疏水性为83.67±1.13。酵母凝集实验表明植物乳杆菌ZJ316的酵母凝集度为32,加入了D-甘露糖和甲基-α-D-甘露糖苷的的实验组,乳酸菌的黏附力下降,说明植物乳杆菌(Lactobacillus plantarum ZJ316)的黏附与特异性黏附中的甘露糖结合相关联。
关键词: 植物乳杆菌 黏附机制 酵母凝集性 表面性质 糖类专一性
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STUDY ON YEAST AGGLUTINATION AND SURFACE CHARACTERISTICS OF LACTOBACILLUS PLANTARUM ZJ316
Abstract:Lactobacillus is the advantage microbial flora in the intestinal of human and animal, the beneficial lactic acid bacteria in organism can regulate microecological balance of intestinal canal in human and animal, restrain and kill pathogenic bacteria, simultaneously, enhance immunity role. Adhesion to the host intestinal is the premise of Lactic acid bacteria exert all kinds of physiological function. The study of adhesion mechanism of lactic acid bacteria has important significance to the development and application of probiotics.In this study,Lactobacillus plantarum ZJ316 is preserved in our laboratory,which isolated from infant fecal samples and produce bacteriocin.we took the microbial adhesive hydrocarbon method (MATH) to survey and evaluatethe surface property of the three lactobacillus (Lactobacillus plantarum ZJ316,Lactobacillus plantarum ZJ22,Enterococcus faecium ZJ19)including hydrophobic property,surface charge and the polymerization ability and the Saccharomyces cerevisiae agglutination experiment to make clear of lactic acid bacteria carbohydrate specificity.Therefore, it lays a good foundation for the study of the adhesion mechanism of lactic acid bacteria.The results showed that the value of hydrophobicity of Lactobacillus plantarum ZJ316 is 83.67±1.13.Yeast agglutination experiments showed that the value of Saccharomyces cerevisiae agglutination was 32.When added in the D-mannose and methyl alpha-D-mannose glucoside to the experimental groupt,the ability of Saccharomyces cerevisiae agglutination declined.It illustrated that the adhesion of Lactobacillus plantarum ZJ316 associated with mannose in specific adhesion.
Keywords: Lactobacillus plantarum adhesion mechanism Yeast agglutination surface property carbohydrate specificity
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No.4733911120016014****
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