Role of lncRNA H19 derived miR-675 in promoting bladder cancer cell proliferation and progression

• 1、The Tenth People's Hospital, Tongji University, Shanghai 200072

Abstract：To detect the expression level of H19, miR-675 and RB in bladder cancer cell lines and tissues adn verify the function of H19/miR-675 in promoting bladder cancer proliferation through down regulating their target gene. we included 3 types of bladder cancer cell lines (T24, 5637, EJ) and normal bladder cell line (SV-HUC-1). Real-time PCR (SYBR Green Ⅰ) was performed to detecting the expression level of Role of lncRNA H19 derived miR-675 in promoting bladder cancer cell proliferation and progressionH19, miR-675 and RB in bladder cancer cell lines and tissues, while Western-blot were performed for detecting the expression level of RB protein in cells and tissues. we selected T24 cell line as study subject, and then transfected T24 cell with H19 pc-DNA and siRNA, detecting the expression level of miR-675 and the ability of cell proliferation and migration; transfected T24 cell with miR-675 mimics and inhibitor, detecting the expression level of RB protein and the ability of cell proliferation and migration; transfected T24 cell with RB pc-DNA and siRNA, detecting the ability of cell proliferation and migration. The results showed that, comparing to normal bladder cell line, the expression levels of H19 and miR-675 are significant increased in bladder cancer cell lines. Meanwhile, comparing to normal bladder tissue, the expression levels of H19 and miR-675 are increased in bladder cancer tissue, the differences had significances (p<0.05). The expression level of RB in bladder cancer tissue was decreased while increased in normal bladder tissue. In this experiment, we have confirmed that the H19/miR-675/RB pathway functions vitally in the formation and developing of the bladder cancer.

Keywords： Urinary bladder cancer LncRNA；H19 Proliferation Tumor marker