Preparation of Atg7 conditional knockout mice in brain microvascular endothelial cells and observation of cerebral microvascular distribution

• 1、China Medical University,Department of developmental cell biology
• 2、China Medical University,institute of translational research

Abstract：Aim Construction of Atg7 conditional knockout mice (Atg7-/-) in brain microvascular endothelial cells and observation of the distribution of cerebral microvessels. Method The mouse tail samples were collected and the mouse tail DNA was extracted by the DNA extraction Kit. The relevant primers were designed and synthesized. PCR and agarose gel electrophoresis were used to analyze the genotypes of mice. The brains from two months and nine months aged mice were collected and frozen sections were used to detect the distribution of vascular markers in wild-type and Atg7-/- mice. Results Atg7-/- mice were successfully constructed. There was no significant difference in cerebral cortical blood vessel density between one months of Atg7-/- mice and wild-type mice. In addition, compared with wild-type mice, the density of cerebral cortical vascular of three months aged Atg7-/- mice was significantly decreased. Conclusion The knockout of Atg7 in cerebral microvascular endothelial cells can decrease the density of cerebral cortical vascular.

Keywords： Atg7 brain microvascular endothelial cell vascular density